کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136712 | 1494022 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies
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کلمات کلیدی
EMAQCHFDAQCLMRMDBSLLOQLC–MS/MS - LC-MS / MSAnti-influenza - آنفلوانزاinternal standard - استاندارد داخلیstandard deviation - انحراف معیارLOD یا Limit of detection - حد تشخیصstandard error of mean - خطای استاندارد میانگینFood and Drug Administration - سازمان غذا و داروCoefficient of Variation - ضریب تغییرmatrix factor - عامل ماتریسpharmacokinetic - فارماکوکینتیکdried blood spots - لکه های خون خشک شدهlimit of detection - محدودیت تشخیصSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیQCM - میکروبازار کریستال کوارتزmultiple reaction monitoring - نظارت چندگانه چندگانهDried blood spot - نقطه خالی خشکquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC-MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20 μL of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue. HPLC separations were run on a reversed-phase microcolumn; the time of analysis was less than 2 min. MS/MS detection was performed on a triple quadrupole mass-spectrometer using multiple reaction monitoring (MRM) mode. Transitions 196.4 â 122.2/153.3 and 152.2 â 93.1/107.2 were monitored for camphecene and 2-adamantylamine hydrochloride (internal standard), respectively. The intra- and inter-day precisions and accuracies, matrix factor, carry-over and recovery were within acceptable limits. Despite low extraction recovery (less than 2%), the sensitivity of the method was enough to detect the analyte in the concentration range 50-2500 ng/mL. The application of the method was shown in pharmacokinetic studies of camphecene in rats at a dose of 10 mg/kg.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1036â1037, 15 November 2016, Pages 136-141
Journal: Journal of Chromatography B - Volumes 1036â1037, 15 November 2016, Pages 136-141
نویسندگان
Artem D. Rogachev, Olga I. Yarovaya, Sergey V. Ankov, Mikhail V. Khvostov, Tatyana G. Tolstikova, Andrey G. Pokrovsky, Nariman F. Salakhutdinov,