کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138366 | 1494601 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid and sensitive UHPLC-MS/MS method for quantification of 83b1 in plasma and its application to bioavailability study in rats
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کلمات کلیدی
CmaxPGHAUC0–tCDDPESCCtmaxMTSMTBEprostaglandin HPPARMRTCOX-2SRMAUC - AUCt1/2 - t1 / 2UHPLC–MS/MS - UHPLC-MS / MSMethod validation - اعتبار سنجی روشapparent volume of distribution - حجم ظاهری توزیعtime to maximum plasma concentration - زمان به حداکثر غلظت پلاسماcisplatin - سیس پلاتینCyclooxygenase-2 - سیکلوکوکسیژناز2Ultra-high performance liquid chromatography tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع با عملکرد بالاBioavailability - فراهم زیستیmethyl tert-Butyl ether - متیل ترتل-بوتیل اترterminal elimination half-life - نیمه عمر حذف ترمینالEsophageal squamous cell carcinoma - کارسینوم سلول سنگفرشی مریquality control - کنترل کیفیتperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Great attentions have been drawn by quinoline for its broad bioactivity as anti-fungal, anti-bacterial and anti-tumor activities. Compared with cisplatin, 83b1, a quinoline derivative, showed equal activity in anti-tumor and lower cyctotoxicity in normal cell. In this study, a simple, rapid and sensitive method for determination of 83b1 in rat plasma using UHPLC-MS/MS was developed for the first time. Loratadine was used as an internal standard (IS). Separation was performed on an Xterra MS C18 column by isocratic elution using acetonitrile: water solution with 1â° formic acid (90:10, v/v) as mobile phase at a flow rate of 0.3 mL/min. A triple quadrupole mass spectrometer operating in the positive ion-switching electron spray ionization mode with selection reaction monitoring (SRM) was employed to determine 83b1 and IS transitions of m/z 321.82 â 147.84, 382.71 â 258.76 for 83b1 and Loratadine, respectively. The values of specificity, linearity and lower limit of quantification, intra- and inter- day precision and accuracy, extraction recovery, matrix effect and stability for this method satisfied the acceptable limits. The lower limit of quantification was 0.5 ng/mL with a linear range of 0.5-1500 ng/mL. The validated method was employed to study the bioavailability of 83b1 in rat by dosing with intravenous injection (1 mg/kg) and gavage (10 mg/kg), and the oral bioavailability of 83b1 in rat was calculated as 20.9 ± 8.8%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 134, 5 February 2017, Pages 71-76
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 134, 5 February 2017, Pages 71-76
نویسندگان
Wen Dingsheng, Guo Jing, Jiang Fulin, Huang Caishun, Zhao Zhenzhen, Lu Gui, Chen Jiangying, Qin Liuyun, Li Zhangwei, Wang Xueding, Deng Zhuoan, Huang Min, Chan Albert Sun Chi, Tang Johnny Cheuk On, Zhong Guoping,