کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5157332 1500592 2017 39 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of N-succinyl chitosan-collagen peptide copolymer and its characterization
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Enzymatic synthesis of N-succinyl chitosan-collagen peptide copolymer and its characterization
چکیده انگلیسی
Collagen peptide (COP) grafted N-succinyl chitosan (NSC) was prepared by using microbial transglutaminase (MTGase) as biocatalyst. The catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The degree of substitution (DS) of N-succinyl chitosan-collagen peptide (NSC-COP)depended on the reaction time, the reaction temperature, the mass ratio of COP to NSC and the mass ratio of MTGase to NSC. NSC-COP showed excellent moisture absorption and retention properties. Antioxidant activities of varying DS and concentration of NSC-COP were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl. Methylthiazol tetrazolium (MTT) assay exhibited that at a suitable concentration NSC-COP with different DS value could promote L929 mouse fibroblasts effectively. The animal experiment indicated that the wound covered with NSC-COP were completely filled with new epithelium within 2 weeks without any significant adverse side reactions. Therefore, the results may contribute to finding the application of NSC-COP in pharmaceutical and biomedical fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 166, 15 June 2017, Pages 45-54
نویسندگان
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