Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions

► Heterologous expression and purification of specifier proteins. ► In vitro characterization on different glucosinolate substrates. ► Effect of ferrous ion and reaction conditions on nitrile-specifier activity.