کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5165692 1502411 2011 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex
چکیده انگلیسی
► We purified near to homogeneity five ficin (iso)forms from Ficus carica latex. ► CD data showed similar secondary and tertiary structures to papain family enzymes. ► Mass spectrometry analyses clearly identified at least four different forms. ► Enzymatic activity measurements suggested different substrate specificities. ► Inhibitory activity confirmed the cysteine proteases nature of all ficin forms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytochemistry - Volume 72, Issues 14–15, October 2011, Pages 1718-1731
نویسندگان
, , , , , ,