A dynamic role for sterols in embryogenesis of Pisum sativum

► GC-MS analysis of 25 steroids in Pisum sativum demonstrates dynamic sterol biosynthesis. ► Early stage embryos contain high sterol concentrations in comparison to other tissues. ► Isofucosterol levels are elevated while stigmasterol levels are low in young embryos. ► Coordinate transcript expression of sterol biosynthesis genes regulates sterol levels.