Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile

We measured ESP activity over 15 days of broccoli seedling development and correlated these with myrosinase activity and the levels of terminal alkenyl glucosinolates and sulphoraphane nitrile. We show ESP activity increases to day 2 before decreasing to imbibed seed activity at day 5. Terminal alkenyl glucosinolate and sulforaphane nitrile content and ESP activity correlated closely.