Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious)

Immunoblot and immunolocalization analysis identified a 47.9 kDa alcohol acyltransferase from apple, encoded by the MdAAT2 gene, and localized mainly in peel tissue. The regulatory mechanism of MdAAT2 in ester biosynthesis was also investigated at mRNA, protein and enzyme activity levels.