Effect of resveratrol on platelet aggregation by fibrinogen protection

- This study shows the ability of resveratrol to preserve fibrinogen from denaturation and platelets from aggregation.
- SEM analysis revealed that the presence of resveratrol completely inhibited the platelet aggregation.
- The degree of platelet adhesion decreased by increasing the resveratrol concentration.
- The protective properties of resveratrol can be explained on the basis its ability to bind the catecholamine.

The effect of resveratrol (RSV) in inhibiting platelet adhesion and aggregation, as well as fibrinogen (FBG) conformational changes promoted by epinephrine (EP), were studied, by using complementary experimental techniques.NMR and IR spectroscopies were used to investigate possible protective effects by RSV towards FBG, in presence of EP. The protective effect of RSV towards FBG was highlighted by spin nuclear relaxation experiments that were interpreted for determining the thermodynamic equilibrium constants of FBG-EP interaction, and by infrared measurements, that showed EP-induced conformational changes of FBG.The ability of RSV in inhibiting platelet adhesion and aggregation promoted by EP was evaluated by scanning electron microscopy (SEM), measuring the platelet adhesion and aggregation degree, in comparison to data obtained for platelet aggregation in platelet rich plasma (PRP).The experimental combined approach pointed out that RSV is able to protect both FBG and platelets from the denaturant and aggregating action of EP at stress level concentration.

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