کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5372020 | 1503974 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Predicting ultraviolet spectrum of single stranded and double stranded deoxyribonucleic acids Predicting ultraviolet spectrum of single stranded and double stranded deoxyribonucleic acids](/preview/png/5372020.png)
Synthetic oligodeoxynucleotides are widely used in many biological, biochemical and biophysical applications. The concentration, composition and structure of DNA are often determined from its ultraviolet spectrum. Although parameters for use with the nearest-neighbor model for prediction of extinction coefficients of single stranded DNAs at 260Â nm were published some time ago, similar parameters for other wavelengths or for use with DNA duplexes have not been reported. Practical formulae and parameters for prediction of UV spectra, hypochromism and peak wavelengths were experimentally determined for both single stranded and double stranded oligodeoxynucleotides in the range from 215 to 310Â nm. The accuracy of predictions made using the nearest-neighbor model and the base composition model was determined and compared. The spectrum of any DNA oligomer can be calculated using a Microsoft Excel® application that is available in the Appendix A.
Journal: Biophysical Chemistry - Volume 133, Issues 1â3, March 2008, Pages 66-70