کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5399778 | 1505906 | 2014 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An insight into the binding between ester-functionalized cationic Gemini surfactant and lysozyme
ترجمه فارسی عنوان
نگاهی به اتصال بین سریم آلیاژی کاتیونی استر و لیزوزیم با عامل استرپتوزیس
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
چکیده انگلیسی
The interactions of cationic dodecyl betainate gemini (DBG) surfactant with lysozyme was studied by fluorescence, time resolved fluorescence, UV-visible, circular dichroism, and molecular docking methods. The results showed that the DBG quenched the fluorescence of lysozyme through static quenching mechanism as confirmed by time resolved spectroscopy. The Stern-Volmer quenching constant (Ksv) and relevant thermodynamic parameters such as enthalpy change (ÎH), Gibbs free energy change (ÎG) and entropy change (ÎS) for interaction system were calculated at different temperatures. The results revealed that hydrophobic forces played a major role in the interactions process. The results of synchronous fluorescence, UV-visible and CD spectra demonstrated that the binding of DBG with lysozyme induces conformational changes in lysozyme. Moreover, the molecular modeling results shows the possible binding sites in the interaction system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 154, October 2014, Pages 298-304
Journal: Journal of Luminescence - Volume 154, October 2014, Pages 298-304
نویسندگان
Rajan Patel, Jitendra Kumar Maurya, Muzaffar Ul Hassan Mir, Meena Kumari, Neha Maurya,