کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5400258 1505911 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex
چکیده انگلیسی
The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 149, May 2014, Pages 185-189
نویسندگان
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