NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction

▸ Interaction with HSA of 20 common blood plasma metabolites is investigated by NMR. ▸ Most of these metabolites bind to HSA, but are released by fatty acids. ▸ Competition for HSA binding is absent for most of these metabolites. ▸ Protein removal by ultrafiltration does not remove metabolite, citrate excepted. ▸ These mappings provide leads to improved NMR metabolic profiling of blood plasma.