کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5477635 | 1399229 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening of bacterial strains isolated from uranium mill tailings porewaters for bioremediation purposes
ترجمه فارسی عنوان
غربالگری سویه های باکتریایی جدا شده از حفره های آسیاب اورانیوم برای دفع زباله
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کلمات کلیدی
EDXHRTEMBZnSTEM-HAADFBrUPorewaters - آبهای زیرزمینیenergy dispersive x-ray - اشعه ایکس پراکنده انرژیUranium - اورانیومBacterial isolates - جدایه های باکتریاییBioremediation - زیست پالاییhigh resolution transmission electron microscope - میکروسکوپ الکترونی با وضوح بالاBiomineralization - کانی سازی زیستی یا بیومینرالیزاسیون
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی هسته ای و مهندسی
چکیده انگلیسی
The present work characterizes at different levels a number of bacterial strains isolated from porewaters sampled in the vicinity of two French uranium tailing repositories. The 16S rRNA gene from 33 bacterial isolates, corresponding to the different morphotypes recovered, was almost fully sequenced. The resulting sequences belonged to 13 bacterial genera comprised in the phyla Firmicutes, Actinobacteria and Proteobacteria. Further characterization at physiological level and metals/metalloid tolerance provided evidences for an appropriate selection of bacterial strains potentially useful for immobilization of uranium and other common contaminants. By using High Resolution Transmission Electron Microscope (HRTEM), this potential ability to immobilize uranium as U phosphate mineral phases was confirmed for the bacterial strains Br3 and Br5 corresponding to Arthrobacter sp. and Microbacterium oxydans, respectively. Scanning Transmission Electron Microscope- High-Angle Annular Dark-Field (STEM-HAADF) analysis showed U accumulates on the surface and within bacterial cytoplasm, in addition to the extracellular space. Energy Dispersive X-ray (EDX) element-distribution maps demonstrated the presence of U and P within these accumulates. These results indicate the potential of certain bacterial strains isolated from porewaters of U mill tailings for immobilizing uranium, likely as uranium phosphates. Some of these bacterial isolates might be considered as promising candidates in the design of uranium bioremediation strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Environmental Radioactivity - Volume 166, Part 1, January 2017, Pages 130-141
Journal: Journal of Environmental Radioactivity - Volume 166, Part 1, January 2017, Pages 130-141
نویسندگان
Iván Sánchez-Castro, Ahinara Amador-GarcÃa, Cristina Moreno-Romero, Margarita López-Fernández, Vannapha Phrommavanh, Jeremy Nos, Michael Descostes, Mohamed L. Merroun,