کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5478335 | 1521809 | 2017 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interactive effects of light and temperature on pigments and n-3 LC-PUFA-enriched oil accumulation in batch-cultivated Pavlova lutheri using high-bicarbonate supply
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کلمات کلیدی
EPASFAMUFATChlTFAChlGC-FIDn-3 LC-PUFATCARAFDWPDATomega-3 long-chain polyunsaturated fatty acidsash-free dry weightROS - ROSβ-car - β ماشینβ-carotene - β-کاروتنEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکsaturated fatty acids - اسیدهای چرب اشباع شدهmonounsaturated fatty acids - اسیدهای چرب غیر اشباعstandard deviation - انحراف معیارTriacylglycerols - ترشیال گلیسیرینTAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولDHA - دوکوساهگزائنوئیک اسیدDiatoxanthin - دیاتوکسانتینDiadinoxanthin - دیادینوکسانتینChlorophyll - سبزینه یا کلروفیلphospholipid:diacylglycerol acyltransferase - فسفولیپید: دی سیل گلیسرول آسیل ترانسفرازFucoxanthin - فوکوکسانینPolar lipids - لیپید های قطبیfatty acid methyl esters - متیل استرهای اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربNitrogen - نیتروژنPavlova lutheri - پاولوا لوتریTotal fatty acids - کل اسیدهای چربTotal carotenoids - کل کاروتنوئیدهاGas Chromatography-Flame Ionization Detector - گاز کروماتوگرافی-شعله یونیزاسیون آشکارسازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Interactive effects of light and temperature on pigments and n-3 LC-PUFA-enriched oil accumulation in batch-cultivated Pavlova lutheri using high-bicarbonate supply Interactive effects of light and temperature on pigments and n-3 LC-PUFA-enriched oil accumulation in batch-cultivated Pavlova lutheri using high-bicarbonate supply](/preview/png/5478335.png)
چکیده انگلیسی
We investigated the simultaneous effects of light and temperature on pigments, lipid remodeling, and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) partitioning into lipid classes of P. lutheri. Biomass was produced in batch cultivation using high bicarbonate supply, and hence, any potential accumulation of lipid and triacylglycerols (TAG) containing n-3 LC-PUFA was triggered by nitrogen (N) limitation. The maximum productivities of both eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were obtained after nitrate-depletion when P. lutheri was cultivated at optimal growth conditions; these occurred at an intermediate temperature of 18 °C and low light intensity (40 μmol photons·mâ 2·sâ 1) for EPA, but there was no impact of light (40 or 200 μmol photons·mâ 2·sâ 1) for DHA production. Under these respective optimum conditions, TAG accounted for 82-84% of TFA on day 15 of cultivation, which contained up to 76-84% of the total cellular EPA and 67-81% of total cellular DHA. Our results clearly demonstrate the process of lipid remodeling in P. lutheri and, for the first time, a potential membrane lipid turnover with transfer of n-3 LC-PUFA (EPA and DHA) from membranes (polar lipids, PL) to storage lipids (TAG), highlighting the accumulation of n-3 LC-PUFA-rich oil during N-starvation (i.e., TAG containing: EPA ~ 12-13% and DHA ~ 5-6% of TFA). When cultivated under low light conditions, P. lutheri additionally accumulated substantial quantities of antioxidant pigments (i.e., fucoxanthin, diadinoxanthin and β-carotene), which add value to extracted bioactive oil for functional food applications. Omega-3 rich oil accumulation and pigment levels in P. lutheri appear to be simultaneously regulated by both light and temperature, in addition to N-limitation during batch-cultivation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 23, April 2017, Pages 113-125
Journal: Algal Research - Volume 23, April 2017, Pages 113-125
نویسندگان
Freddy Guihéneuf, Dagmar B. Stengel,