کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5501868 | 1534942 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Yap1 and Skn7 genetically interact with Rad51 in response to oxidative stress and DNA double-strand break in Saccharomyces cerevisiae
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کلمات کلیدی
DSB2',7'-dichlorodihydrofluorescein diacetatePhleomycinYAP1SSBPHLMMSBERGFPNHEJNER - DOWNH2DCFDA - H2DCFD بهROS - ROSnucleotide excision repair - تعمیر مجدد نوکلئوتیدیbase excision repair - تعمیر پایه پایهGenome stability - ثبات ژنومRad51 - رادیلا 51double-strand break - شکست دو ردیفsingle-strand break - شکستن تک رشتهnon-homologous end joining - عدم پیوستن انتهای غیر همولوگmethyl methanesulfonate - متیل متان سولفوناتHomologous recombination - نوترکیبی همولوگHydroxyurea - هیدروکسی اورهParaquat - پاراکواتgreen fluorescent protein - پروتئین فلورسنت سبزReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Reactive oxygen species (ROS)-mediated DNA adducts as well as DNA strand breaks are highly mutagenic leading to genomic instability and tumorigenesis. DNA damage repair pathways and oxidative stress response signaling have been proposed to be highly associated, but the underlying interaction remains unknown. In this study, we employed mutant strains lacking Rad51, the homolog of E. coli RecA recombinase, and Yap1 or Skn7, two major transcription factors responsive to ROS, to examine genetic interactions between double-strand break (DSB) repair proteins and cellular redox regulators in budding yeast Saccharomyces cerevisiae. Abnormal expression of YAP1 or SKN7 aggravated the mutation rate of rad51 mutants and their sensitivity to DSB- or ROS-generating reagents. Rad51 deficiency exacerbated genome instability in the presence of increased levels of ROS, and the accumulation of DSB lesions resulted in elevated intracellular ROS levels. Our findings suggest that evident crosstalk between DSB repair pathways and ROS signaling proteins contributes to cell survival and maintenance of genome integrity in response to genotoxic stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 101, December 2016, Pages 424-433
Journal: Free Radical Biology and Medicine - Volume 101, December 2016, Pages 424-433
نویسندگان
Dae Gwan Yi, Myung Ju Kim, Ji Eun Choi, Jihyun Lee, Joohee Jung, Won-Ki Huh, Woo-Hyun Chung,