کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505072 | 1400260 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet
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کلمات کلیدی
CCAAT-enhancer-binding proteinsPPARγAP2GLUT4FOXO1OGTTIWATPI3KAS160Akt substrate of 160 kDaAUC - AUCC/EBPα - C / EBPαH&E - H & EPI3K/AKT - PI3K / AKTAdipogenesis - آدیپوژنزinsulin tolerance test - آزمون تحمل انسولینOral glucose tolerance test - آزمون تحمل گلوکز خوراکیAkt - آکتITT - اینجاGlucose tolerance - تحمل گلوکزtriglyceride - تریگلیسریدglucose transporter 4 - حمل کننده گلوکز 4Phosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازPhytol - فیتولarea under the curve - منطقه تحت منحنیMice - موشHematoxylin and Eosin - هماتوکسیلین و ائوزینwortmannin - ورتمنینadipocyte protein 2 - پروتئین آدیپوسیت 2Forkhead box protein O1 - پروتئین جعبه ی جعبه ای O1protein kinase B - پروتئین کیناز Bperoxisome proliferator-activated receptor γ - گیرنده پروتئین کننده پروکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been shown that adipose tissue hyperplasia (increased adipocyte number or adipogenesis) has beneficial effects on metabolic health. The aim of the present study was to determine whether phytol could modulate hyperplasia/adipogenesis and glucose homeostasis, and to explore the underlying mechanisms in mice fed high-fat and high fructose diet (HFFD). Our results demonstrated that phytol administration decreased body weight gain and inguinal subcutaneous white adipose tissue (iWAT) weight. However, phytol significantly increased the adipocyte number in iWAT, with the smaller average adipocyte diameter. Meanwhile, OGTT result showed that phytol improved glucose tolerance. In accord, phytol administration markedly increased expression of marker genes associated with adipogenesis (PPARγ and C/EBPα) and glucose uptake (AS160 and GLUT4) and activated PI3K/Akt signaling pathway in mice iWAT. In agreement with the in vivo findings, the in vitro results indicated that 100 μM phytol significantly enhanced 3T3-L1 adipogenesis and glucose uptake, and activated PI3K/Akt signaling pathway. However, phytol-induced enhancement of 3T3-L1 adipognesis and glucose uptake, activation of PI3K/Akt signaling pathway, elevation of marker genes involved in adipogensis and glucose uptake, as well as translocation of GLUT4 from cytoplasm to membrane were abolished by Wortmannin, a specific PI3K/Akt inhibitor. Taken together, phytol increased adipocyte number in iWAT and improved glucose tolerance in mice fed HFFD, which was coincident with the enhanced adipogenesis and glucose uptake in 3T3-L1, and was associated with activation of PI3K/Akt signaling pathway. These data suggested the application of phytol as a potential nutritional agent to combat obesity and type 2 diabetes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 489, Issue 4, 5 August 2017, Pages 432-438
Journal: Biochemical and Biophysical Research Communications - Volume 489, Issue 4, 5 August 2017, Pages 432-438
نویسندگان
Jianbing Wang, Xiaoquan Hu, Wei Ai, Fenglin Zhang, Kelin Yang, Lina Wang, Xiaotong Zhu, Ping Gao, Gang Shu, Qingyan Jiang, Songbo Wang,