کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5507737 | 1400345 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selective regulation of biological processes by vitamin D based on the spatio-temporal cistrome of its receptor
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کلمات کلیدی
FDRdirect repeat spaced by 3 nucleotides1,25(OH)2D3 or 1,25DDR3FAIRE-seqCTCFVDRMACSIGVTSSRNA-seqChIP-Seq1α,25-dihydroxyvitamin D3 - 1α، 25-دی هیدروکسوییتامین D3SOM - WHOchromatin immunoprecipitation - ایمن سازی کروماتینTAD - بلهRNA sequencing - ترتیب RNAChIP sequencing - ترتیب چیپfold change - تغییر در برابرformaldehyde-assisted isolation of regulatory elements sequencing - جداسازی فرمالدئید از عناصر تنظیم کننده عناصرtopologically associated domain - دامنه مرتبط با توپولوژیtranscription start site - رونویسی شروع سایتCCCTC-binding factor - عامل اتصال دهنده CCCTCIntegrative Genomics Viewer - مجتمع ژنومیک بینندهfalse discovery rate - میزان کشف کاذبSelf-organizing map - نقشه خودسازماندهGene ontology - هستیشناسی ژنیHomer - هومرCHiP - چیپGorilla - گوریلVitamin D receptor - گیرنده ویتامین D
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The transcription factor vitamin D receptor (VDR) is the exclusive nuclear target of the biologically active form of vitamin D (1,25(OH)2D3). In THP-1 human monocytes we obtained a highly accurate VDR cistrome after 2 and 24Â h ligand stimulation comprising >Â 11,600 genomic loci, 78% of which were detected exclusively after 24Â h. In contrast, a group of 510 persistent VDR sites occurred at all conditions and some 2100 VDR loci were only transiently occupied. Machine learning and statistical analysis as well as a comparison with the re-analyzed B cell VDR cistrome indicated a subgroup of 339 highly conserved persistent VDR sites that were suited best for describing vitamin D-triggered gene regulatory scenarios. The 1,25(OH)2D3-dependent transcriptome of THP-1 cells comprised 587 genes, 311 of which were primary targets with main functions in the immune system. More than 97% of the latter genes were located within 1,25(OH)2D3-modulated topologically associated domains (TADs). The number of persistent and transient VDR sites was found to be the main discriminator for sorting these TADs into five classes carrying vitamin D target genes involved in distinct biological processes. In conclusion, specific regulation of biological processes by vitamin D depends on differences in time-dependent VDR binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1860, Issue 9, September 2017, Pages 952-961
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1860, Issue 9, September 2017, Pages 952-961
نویسندگان
Antonio Neme, Sabine Seuter, Carsten Carlberg,