کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5508486 | 1537694 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Epoxyalcohol synthase of Ectocarpus siliculosus. First CYP74-related enzyme of oxylipin biosynthesis in brown algae
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کلمات کلیدی
TMSOxylipins(9Z,11E,13S)-13-hydro(pero)xy-9,11-octadecadienoic acidHPLIMAC13-H(P)OD9-H(P)ODEASHSQCNOESYtrimethylsilyl(9S,10E,12Z)-9-hydro(pero)xy-10,12-octadecadienoic acidNuclear Overhauser Effect SpectroscopY - Spectroscopy اثر Overhauser هسته ایDES - ازAOS - بهDivinyl ether synthase - دی دیینیل اتر سنتازEpoxyalcohol synthase - سنتاز اپوکسی الکلAllene oxide synthase - سنتاز اکسید آلنCytochrome P450 - سیتوکروم پی۴۵۰SIM - سیم کارتHeteronuclear single quantum coherence spectroscopy - طیف سنجی تک هسته ای تک هسته ای کوانتومیcorrelation spectroscopy - طیف سنجی همبستگیheteronuclear multiple-bond correlation spectroscopy - طیف سنجی همبستگی چند باند هترونیکی هسته ایMethyl - متیلselected ion monitoring - نظارت بر یون انتخاب شدهHMBC یا Heteronuclear Multiple Bond Correlation - همبستگی پیوند چندگانه ناجورهستهHydroperoxide lyase - هیدروپراکسید لیازCOSY - کثیفimmobilized metal affinity chromatography - کروماتوگرافی وابسته به فلز متمرکز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enzymes of CYP74 family play the central role in the biosynthesis of physiologically important oxylipins in land plants. Although a broad diversity of oxylipins is known in the algae, no CYP74s or related enzymes have been detected in brown algae yet. Cloning of the first CYP74-related gene CYP5164B1 of brown alga Ectocarpus siliculosus is reported in present work. The recombinant protein was incubated with several fatty acid hydroperoxides. Linoleic acid 9-hydroperoxide (9-HPOD) was the preferred substrate, while linoleate 13-hydroperoxide (13-HPOD) was less efficient. α-Linolenic acid 9- and 13-hydroperoxides, as well as eicosapentaenoic acid 15-hydroperoxide were inefficient substrates. Both 9-HPOD and 13-HPOD were converted into epoxyalcohols. For instance, 9-HPOD was turned primarily into (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid. Both epoxide and hydroxyl oxygen atoms of the epoxyalcohol were incorporated mostly from [18O2]9-HPOD. Thus, the enzyme exhibits the activity of epoxyalcohol synthase (EsEAS). The results show that the EsEAS isomerizes the hydroperoxides into epoxyalcohols via epoxyallylic radical, a common intermediate of different CYP74s and related enzymes. EsEAS can be considered as an archaic prototype of CYP74 family enzymes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1862, Issue 2, February 2017, Pages 167-175
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1862, Issue 2, February 2017, Pages 167-175
نویسندگان
Yana Y. Toporkova, Valeria S. Fatykhova, Yuri V. Gogolev, Bulat I. Khairutdinov, Lucia S. Mukhtarova, Alexander N. Grechkin,