کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5520434 1544900 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improvement in biochemical characteristics of glycosylated phytase through immobilization on nanofibers
ترجمه فارسی عنوان
بهبود ویژگی های بیوشیمیایی فیتاز گلیکوزیل شده از طریق بی حرکت شدن روی نانوسایل
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


- A response surface method was used to obtain the best parameters for enzyme immobilization.
- Improvement in kinetics characteristics of phytase by immobilization on nanofibrous membrane.
- Performance of in silico analysis to predict the Enzyme-nanofiber interaction.

The objective of the current study was to enzymatically characterize immobilized phytase as a glycoprotein on an optimal blend of starch/PAAm nanofibers. Aspergillus niger phytase (phyA) was first expressed in Hansenula polymorpha using formate dehydrogenase promoter and α-mating factor signal peptide. Response surface methodology was then used to optimize the different levels of the enzyme, temperature and pH on the immobilized phytase. The electrospun nanofibers showed uniform morphology with an average diameter of approximately 269 nm. Afterward, the kinetic parameters of Km (56 μM) and Vmax (401 μmol/min mg) of the immobilized enzyme improved, but the optimum pH and temperature had not changed in comparison with the soluble phytase. The applicability of the nanofiber was confirmed by its outstanding immobilization efficiency (193%) and suitable retention of catalytic activity of the immobilized phytase (50.4% after the tenth repetition). Optimal orientation of the enzyme on the nanofiber using the in silico approach was predicted, which could improve the kinetic properties. The results demonstrate the good potential of H. polymorpha as an efficient expression system for the production of glycoproteins for immobilization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 12, October 2017, Pages 96-103
نویسندگان
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