کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5526906 1401555 2017 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineered cartilage regeneration from adipose tissue derived-mesenchymal stem cells: A morphomolecular study on osteoblast, chondrocyte and apoptosis evaluation
ترجمه فارسی عنوان
بازسازی غضروف های مهندسی شده از سلول های بنیادی مزانشیمی مشتق شده از بافت چربی: مطالعه ی مورفومولکولی در ارزیابی استئوآلبوز، کندروسیت و آپوپتوز
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


- Articular cartilage has poor regenerative capacity.
- Use of MSCs for cartilage tissue regeneration is very promising.
- MSCs-derived chondrocytes cultured on CCC constitute a healthy hyaline cartilage.
- RUNX2, osteocalcin, col (I, II), lubricin and caspase-3 expression was assessed.

The poor self-repair capacity of cartilage tissue in degenerative conditions, such as osteoarthritis (OA), has prompted the development of a variety of therapeutic approaches, such as cellular therapies and tissue engineering based on the use of mesenchymal stem cells (MSCs). The aim of this study is to demonstrate, for the first time, that the chondrocytes differentiated from rat adipose tissue derived-MSCs (AMSCs), are able to constitute a morphologically and biochemically healthy hyaline cartilage after 6 weeks of culture on a Collagen Cell Carrier (CCC) scaffold. In this study we evaluated the expression of some osteoblasts (Runt-related transcription factor 2 (RUNX2) and osteocalcin), chondrocytes (collagen I, II and lubricin) and apoptosis (caspase-3) biomarkers in undifferentiated AMSCs, differentiated AMSCs in chondrocytes cultured in monolayer and AMSCs-derived chondrocytes seeded on CCC scaffolds, by different techniques such as immunohistochemistry, ELISA, Western blot and gene expression analyses. Our results showed the increased expression of collagen II and lubricin in AMSCs-derived chondrocytes cultured on CCC scaffolds, whereas the expression of collagen I, RUNX2, osteocalcin and caspase-3 resulted decreased, when compared to the controls. In conclusion, this innovative basic study could be a possible key for future therapeutic strategies for articular cartilage restoration through the use of CCC scaffolds, to reduce the morbidity from acute cartilage injuries and degenerative joint diseases.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 357, Issue 2, 15 August 2017, Pages 222-235
نویسندگان
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