کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5537157 | 1402323 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Manipulation of neuraminidase packaging signals and hemagglutinin residues improves the growth of A/Anhui/1/2013 (H7N9) influenza vaccine virus yield in eggs
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کلمات کلیدی
CVVcandidate vaccine virusVaccine virusMDCKORFTIVNCRRT-PCRMadin-Darby canine kidney cells - سلول های کلیوی کانین Madin-Darbyopen reading frame - قاب خواندن بازNon-coding region - منطقه غیر کدگذاریhemagglutination inhibition - مهار هموگلوبینneuraminidase - نورآمینیدازwild-type - نوع وحشیNucleotide - نوکلئوتیدhemagglutinin - هماگلوتینینplaque-forming unit - واحد پلاک سازیTrivalent influenza vaccine - واکسن سه واکنش آنفلوانزاreverse transcription–polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسpfu - پفو
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In 2013, a novel avian-origin H7N9 influenza A virus causing severe lower respiratory tract disease in humans emerged in China, with continued sporadic cases. An effective vaccine is needed for this virus in case it acquires transmissibility among humans; however, PR8-based A/Anhui/1/2013 (Anhui/1, H7N9), a WHO-recommended H7N9 candidate vaccine virus (CVV) for vaccine production, does not replicate well in chicken eggs, posing an obstacle to egg-based vaccine production. To address this issue, we explored the possibility that PR8's hemagglutinin (HA) and neuraminidase (NA) packaging signals mediate improvement of Anhui/1 CVV yield in eggs. We constructed chimeric HA and NA genes having the coding region of Anhui/1 HA and NA flanked by the 5â² and 3â² packaging signals of PR8's HA and NA, respectively. The growth of CVVs containing the chimeric HA was not affected, but that of those containing the chimeric NA gene grew in embryonated chicken eggs with a more than 2-fold higher titer than that of WT CVV. Upon 6 passages in eggs further yield increase was achieved although this was not associated with any changes in the chimeric NA gene. The HA of the passaged CVV, did, however, exhibit egg-adaptive mutations and one of them (HA-G218E) improved CVV growth in eggs without significantly changing antigenicity. The HA-G218E substitution and a chimeric NA, thus, combine to provide an Anhui/1 CVV with properties more favorable for vaccine manufacture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 35, Issue 10, 7 March 2017, Pages 1424-1430
Journal: Vaccine - Volume 35, Issue 10, 7 March 2017, Pages 1424-1430
نویسندگان
Subrata Barman, Petr S. Krylov, Jasmine C. Turner, John Franks, Robert G. Webster, Matloob Husain, Richard J. Webby,