کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5537722 | 1402357 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeting dendritic cells in humanized mice receiving adoptive T cells via monoclonal antibodies fused to Flu epitopes
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کلمات کلیدی
FACSmAbAPCsPBMCsDCsHPCs - HPC هاMonoclonal antibody - آنتی بادی مونوکلونالAntigen presenting cells - آنتیژن ارائه سلولAdoptive transfer - انتقال واجبfluorescent activated cell sorting - دسته بندی سلول فعال فلورسنت فعال استcytotoxic T cells - سلول های T سیتوتوکسیکperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیHuman dendritic cells - سلول های دندریتی انسانیDendritic cells - سلول های دندریتیکConventional - مرسومHumanized mice - موش های انسانیPlasmacytoid - پلاسماسوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The targeting of vaccine antigens to antigen presenting cells (APC), such as dendritic cells (DCs), is a promising strategy for boosting vaccine immunogenicity and, in turn, protective and/or therapeutic efficacy. However, in vivo systems are needed to evaluate the potential of this approach for testing human vaccines. To this end, we examined human CD8+ T-cell expansion to novel DC-targeting vaccines in vitro and in vivo in humanized mice. Vaccines incorporating the influenza matrix protein-1 (FluM1) antigen fused to human specific antibodies targeting different DC receptors, including DEC-205, DCIR, Dectin-1, and CD40, elicited human CD8+ T-cell responses, as defined by the magnitude of specific CD8+ T-cells to the targeted antigen. In vitro we observed differences in response to the different vaccines, particularly between the weakly immunogenic DEC-205-targeted and more strongly immunogenic CD40-targeted vaccines, consistent with previous studies. However, in humanized mice adoptively transferred (AT) with mature human T cells (HM-T), vaccines that performed weakly in vitro (i.e., DEC-205, DCIR, and Dectin-1) gave stronger responses in vivo, some resembling those of the strongly immunogenic CD40-targeted vaccine. These results demonstrate the utility of the humanized mouse model as a platform for studies of human vaccines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 34, Issue 41, 22 September 2016, Pages 4857-4865
Journal: Vaccine - Volume 34, Issue 41, 22 September 2016, Pages 4857-4865
نویسندگان
John P. Graham, Pierre Authie, Chun I. Yu, Sandra M. Zurawski, Xiao-Hua Li, Florentina Marches, Anne-Laure Flamar, Aditi Acharya, Jacques Banchereau, A. Karolina Palucka,