کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5551698 | 1557799 | 2017 | 7 صفحه PDF | دانلود رایگان |
- In vitro assembly and characterisation of a rubella candidate vaccine based on modified plant virus (spherical particles).
- Candidate vaccine induces efficient humoral immune response against rubella.
- Acute and chronic toxicity studies demonstrate biosafety of candidate vaccine.
- Candidate vaccine demonstrated good results and can be considered as a promising safe tool against the rubella virus.
A novel rubella candidate vaccine based on a structurally modified plant virus - spherical particles (SPs) - was developed. SPs generated by the thermal remodelling of the tobacco mosaic virus are promising platforms for the development of vaccines. SPs combine unique properties: biosafety, stability, high immunogenicity and the effective adsorption of antigens. We assembled in vitro and characterised complexes (candidate vaccine) based on SPs and the rubella virus recombinant antigen. The candidate vaccine induced a strong humoral immune response against rubella. The IgG isotypes ratio indicated the predominance of IgG1 which plays a key role in immunity to natural rubella infection. The immune response was generally directed against the rubella antigen within the complexes. We suggest that SPs can act as a platform (depot) for the rubella antigen, enhancing specific immune response. Our results demonstrate that SPs-antigen complexes can be an effective and safe candidate vaccine against rubella.
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Journal: Antiviral Research - Volume 144, August 2017, Pages 27-33