کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5585139 | 1568114 | 2017 | 50 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis
ترجمه فارسی عنوان
توابع همپوشانی تنظیم کننده های سیلوپروتئین استخوانی و پریو فسفات در مدیریت سیمان زایی
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کلمات کلیدی
ANKOPNPDLOdontogenesisBSPENPP1PPIRGDTNAPEctonucleotide pyrophosphatase phosphodiesterase 1HPPqPCRDMEMSmall Integrin-Binding LIgand N-linked GlycoproteinECMDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoArginine-Glycine-Aspartic Acid - آرژینین-گلیسین-اسیدپارشی اسیدTissue-nonspecific alkaline phosphatase - آلکالین فسفاتاز غیر اختصاصی بافتیOsteopontin - استئوپنتینBone - استخوان Polyglutamic acid - اسید polyglutamicEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاSibling - خواهر و برادرPeriodontal ligament - رباط پریودنتالbone sialoprotein - سیلوپروتئین استخوانdental cementum - سیمان دندانExtracellular matrix - ماتریکس خارج سلولیknock-out - ناک اوتhypophosphatasia - هیپوفسفاتزیquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیPeriodontium - پریودنتیمinorganic pyrophosphate - پیرو فسفات معدنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
چکیده انگلیسی
Although acellular cementum is essential for tooth attachment, factors directing its development and regeneration remain poorly understood. Inorganic pyrophosphate (PPi), a mineralization inhibitor, is a key regulator of cementum formation: tissue-nonspecific alkaline phosphatase (Alpl/TNAP) null mice (increased PPi) feature deficient cementum, while progressive ankylosis protein (Ank/ANK) null mice (decreased PPi) feature increased cementum. Bone sialoprotein (Bsp/BSP) and osteopontin (Spp1/OPN) are multifunctional extracellular matrix components of cementum proposed to have direct and indirect effects on cell activities and mineralization. Studies on dentoalveolar development of Bsp knockout (Bspâ/â) mice revealed severely reduced acellular cementum, however underlying mechanisms remain unclear. The similarity in defective cementum phenotypes between Bspâ/â mice and Alplâ/â mice (the latter featuring elevated PPi and OPN), prompted us to examine whether BSP is operating by modulating PPi-associated genes. Genetic ablation of Bsp caused a 2-fold increase in circulating PPi, altered mRNA expression of Alpl, Spp1, and Ank, and increased OPN protein in the periodontia. Generation of a Bsp knock-out (KO) cementoblast cell line revealed significantly decreased mineralization capacity, 50% increased PPi in culture media, and increased Spp1 and Ank mRNA expression. While addition of 2 μg/ml recombinant BSP altered Spp1, Ank, and Enpp1 expression in cementoblasts, changes resulting from this dose were not dependent on the integrin-binding RGD motif or MAPK/ERK signaling pathway. Decreasing PPi by genetic ablation of Ank on the Bspâ/â mouse background reestablished cementum formation, allowing > 3-fold increased acellular cementum volume compared to wild-type (WT). However, deleting Ank did not fully compensate for the absence of BSP. Bspâ/â; Ankâ/â double-deficient mice exhibited mean 20-27% reduced cementum thickness and volume compared to Ankâ/â mice. From these data, we conclude that the perturbations in PPi metabolism are not solely driving the cementum pathology in Bspâ/â mice, and that PPi is more potent than BSP as a cementum regulator, as shown by the ability to override loss of BSP by lowering PPi. We propose that BSP and PPi work in concert to direct mineralization in cementum and likely other mineralized tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 105, December 2017, Pages 134-147
Journal: Bone - Volume 105, December 2017, Pages 134-147
نویسندگان
M. Ao, M.B. Chavez, E.Y. Chu, K.C. Hemstreet, Y. Yin, M.C. Yadav, J.L. Millán, L.W. Fisher, H.A. Goldberg, M.J. Somerman, B.L. Foster,