کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589245 | 1569810 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning, transcriptional profiling, and subcellular localization of signal transducer and activator of transcription 2 (STAT2) ortholog from rock bream, Oplegnathus fasciatus
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کلمات کلیدی
STAT2Brg1Minimum Information for Publication of Quantitative Real-Time PCR ExperimentsISGTyk2ISREISGF3SH2Regulator of Calcineurin 1Rcan1SAVPBSIPNVTFBSp.i.IFNAR1ISAVSalmon alphavirusIFNAR2IFN-stimulated gene factor 3RBIVMBPCREBgDNALPSFBSqPCRPAMPsIL-10Janus kinase - کیناز جانوس cDNA - cDNAComplementary DNA - DNA تکمیلیgenomic DNA - DNA ژنومیBAC - LACAntiviral effect - اثر ضد ویروسیpathogen-associated molecular patterns - الگوهای مولکولی مرتبط با پاتوژنstandard deviation - انحراف معیارinterferon - اینترفرونIFN - اینترفرون هاInterleukin-10 - اینترلوکین 10TAD - بلهGenomic evolution - تکامل ژنومیtyrosine kinase 2 - تیروزین کیناز 2transactivation domain - دامنه ترانسکتاسیونMIQE - دوستانfetal bovine serum - سرم جنین گاوpost infection - عفونت پستIFN-stimulated response element - عنصر پاسخ تحریک شده توسط IFNlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهSrc Homology 2 - همبستگی Src 2quantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیInfectious salmon anemia virus - ویروس آنمی انسانی عفونیInfectious pancreatic necrosis virus - ویروس ناکروز پانکراس عفونیImmune responses - پاسخ های ایمنیmaltose binding protein - پروتئین متصل به مالتوزPoly I:C - پلی I: CPolyinosinic:polycytidylic acid - پلی سونیک: پلیسییدیدیل اسیدneighbor-joining - پیوستن همسایهJAK - چگونهIFN-stimulated gene - ژن تحریک شده توسط IFNBrahma-related gene 1 - ژن مرتبط با برهام 1bacterial artificial chromosome - کروموزوم مصنوعی باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Signal transducer and activator of transcription 2 (STAT2) is a key element that transduces signals from the cell membrane to the nucleus via the type I interferon-signaling pathway. Although the structural and functional aspects of STAT proteins are well studied in mammals, information on teleostean STATs is very limited. In this study, a STAT paralog, which is highly homologous to the STAT2 members, was identified from a commercially important fish species called rock bream and designated as RbSTAT2. The RbSTAT2 gene was characterized at complementary DNA (cDNA) and genomic sequence levels, and was found to possess structural features common with its mammalian counterparts. The complete cDNA sequence was distributed into 24 exons in the genomic sequence. The promoter proximal region was analyzed and found to contain potential transcription factor binding sites to regulate the transcription of RbSTAT2. Phylogenetic studies and comparative genomic structure organization revealed the distinguishable evolution for fish and other vertebrate STAT2 orthologs. Transcriptional quantification was performed by SYBR Green quantitative real-time PCR (qPCR) and the ubiquitous expression of RbSTAT2 transcripts was observed in all tissues analyzed from healthy fish, with a remarkably high expression in blood cells. Significantly (PÂ <Â 0.05) altered transcription of RbSTAT2 was detected after immune challenge experiments with viral (rock bream irido virus; RBIV), bacterial (Edwardsiella tarda and Streptococcus iniae), and immune stimulants (poly I:C and LPS). Antiviral potential was further confirmed by WST-1 assay, by measuring the viability of rock bream heart cells treated with RBIV. In addition, results of an in vitro challenge experiment signified the influence of rock bream interleukin-10 (RbIL-10) on transcription of RbSTAT2. Subcellular localization studies by transfection of pEGFP-N1/RbSTAT2 into rock bream heart cells revealed that the RbSTAT2 was usually located in the cytoplasm and translocated near to the nucleus upon poly I:C administration. Altogether, these findings suggest that RbSTAT2 is involved in various biologically crucial mechanisms, and provides immune protection to the rock bream.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 626, 30 August 2017, Pages 95-105
Journal: Gene - Volume 626, 30 August 2017, Pages 95-105
نویسندگان
S.D.N.K. Bathige, Navaneethaiyer Umasuthan, Thanthrige Thiunuwan Priyathilaka, William Shanthakumar Thulasitha, J.D.H.E. Jayasinghe, Qiang Wan, Bo-Hye Nam, Jehee Lee,