کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589917 | 1569840 | 2017 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipid metabolism in Rhodnius prolixus: Lessons from the genome
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کلمات کلیدی
iPLA2ACSMcPLA2PLA2HSL4CLGPATACSLACSVLsPLA2BrummerELOVLACSSAcyl-CoA synthetasesInsect physiologySecreted PLA2ACSBGCalcium-independent PLA2Fatty acyl-CoA reductaseCPTVLCFAACSFASG3PBMMRT-PCR4-Coumarate:CoA ligase - 4-کومارات: لیگاز CoAPAF-AH - PAF-قphospholipase A2 - آنزیم فسفولیپاز A2 Fatty acid desaturase - اسید لاکتیک اسیدfatty acid synthase - اسید چرب سنتازFree fatty acids - اسیدهای چرب آِزادFatty acid elongase - اسیدهای چرب اشباع شدهvery long-chain fatty acids - اسیدهای چرب بسیار زنجیره ایFAD - بدFAR - بعداBioinformatics - بیوانفورماتیک Lipid biosynthesis - بیوسنتز لیپیدTAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولtriglyceride - تریگلیسریدGene family - خانواده ژنیdiacylglycerol - دیسیل گلیسیرینDAG - روزPlatelet activating factor acetylhydrolase - عامل فعال کننده پلاکت استیل هیدرولازPhospholipid - فسفولیپیدhormone-sensitive lipase - لیپاز حساس به هورمونMetabolic pathway - مسیر سوخت و سازCarnitine palmitoyltransferase - کارنتین پالمیتیل ترانسفرازglycerol-3-phosphate - گلیسرول 3-فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The kissing bug Rhodnius prolixus is both an important vector of Chagas' disease and an interesting model for investigation into the field of physiology, including lipid metabolism. The publication of this insect genome will bring a huge amount of new molecular biology data to be used in future experiments. Although this work represents a promising scenario, a preliminary analysis of the sequence data is necessary to identify and annotate the genes involved in lipid metabolism. Here, we used bioinformatics tools and gene expression analysis to explore genes from different genes families and pathways, including genes for fat breakdown, as lipases and phospholipases, and enzymes from β-oxidation, fatty acid metabolism, and acyl-CoA and glycerolipid synthesis. The R. prolixus genome encodes 31 putative lipase genes, including 21 neutral lipases and 5 acid lipases. The expression profiles of some of these genes were analyzed. We were able to identify nine phospholipase A2 genes. A variety of gene families that participate in fatty acid synthesis and modification were studied, including fatty acid synthase, elongase, desaturase and reductase. Concerning the synthesis of glycerolipids, we found a second isoform of glycerol-3-phosphate acyltransferase that was ubiquitously expressed throughout the organs. Finally, all genes involved in fatty acid β-oxidation were identified, but not a long-chain acyl-CoA dehydrogenase. These results provide fundamental data to be used in future research on insect lipid metabolism and its possible relevance to Chagas' disease transmission.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 596, 5 January 2017, Pages 27-44
Journal: Gene - Volume 596, 5 January 2017, Pages 27-44
نویسندگان
David Majerowicz, Gustavo M. Calderón-Fernández, Michele Alves-Bezerra, Iron F. De Paula, LÃvia S. Cardoso, M. Patricia Juárez, Georgia C. Atella, Katia C. Gondim,