کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589963 | 1570075 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative genomics and phylogenomics of Trichostrongyloidea mitochondria reveal insights for molecular diagnosis and evolutionary biology of nematode worms
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کلمات کلیدی
PcGND3ND1tRNAND2cox3atp6cytBND6ND5GTRITS-2ITS-1cox2COXRDNArRNACOX1ND4LRibosomal DNA - DNA ریبوزومیMitochondrial DNA - DNA میتوکندریاRibosomal RNA - RNA Ribosomaltransfer RNA - انتقال RNAbase pairs - جفت پایهinternal transcribed spacer 1 - داخلی رونویسی spacer 1Internal transcribed spacer 2 - داخلی رونویسی spacer 2mtDNA - دیانای میتوکندریاییMitochondrion - میتوکندریونpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCytochrome b gene - ژن Cytochrome bProtein Coding Gene - ژن کدگذاری پروتئینMitochondrial genome - ژنوم میتوکندریابی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Haemonchus placei (Nematoda:Trichostrongyloidea:Haemonchidae) is a parasite of cattle and other ruminants that reduces the weight gain and increases mortality causing significant economic losses to livestock worldwide. Here we sequenced and annotated its complete mitochondrion (mt) genome, obtaining a circular molecule of 13,664Â bp long and 78.8% AÂ +Â T content (KT955899). Besides, all 10Â mt genomes publicly available for the Trichostrongyloidea superfamily were downloaded from GenBank database. In the comparison between the complete mt genome sequences of the 3 species from the Haemonchidae family, an AT-rich region of variable size was observed. A primer pair that amplifies this region was proposed to allow the molecular diagnostic and differentiation between H. placei and H. contortus infections. Furthermore, the evolutionary relationship between H. placei and other members of Trichostrongyloidea was inferred based on a phylogenomics approach. All mitochondrial gene sequences were aligned and then concatenated into a supermatrix dataset. A maximum likelihood tree was produced and confirmed the monophyly of Haemonchidae family. The superfamily Trichostrongyloidae was also recovered as a monophyletic clade. The complete mt genome sequence reported here may be used to develop new genetic markers and investigate phylogenetic and geographic relationships between isolates as well as to assess population diversity and allow health control programs monitoring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 9, December 2017, Pages 65-73
Journal: Gene Reports - Volume 9, December 2017, Pages 65-73
نویسندگان
Livia Loiola dos Santos, Francisco Prosdocimi, Nicholas Costa Barroso Lima, Igor Rodrigues da Costa, Danielle Cunha Cardoso, Marcela Gonçalves Drummond, Bruno dos Santos Alves Figueiredo Brasil, Eduardo Bastianetto, Denise Aparecida Andrade de Oliveira,