کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590631 | 1570154 | 2017 | 50 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA expression profiling of primary sheep testicular cells in response to bluetongue virus infection
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کلمات کلیدی
PBSKEGG Orthology Based Annotation SystemBTVdsRNAsnoRNAsnRNAqRT-PCRSOAPCPEFBSMOI - MERNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsmall nuclear RNA - RNA هسته ای کوچکsmall RNA - RNA کوچکsmall nucleolar RNA - RNA کوچک هسته ایsiRNA - siRNACytopathic effect - اثر سیتوپاتیکRNA sequencing - ترتیب RNAKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو microRNAs - ریز آرانایfetal bovine serum - سرم جنین گاوSRNA - سرناMEM - مامانModified Eagle's Medium - متوسط اصلاح شده عقابPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAGene ontology - هستیشناسی ژنیplaque-forming units - واحدهای پلاک سازیquantitative reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزا معکوسBluetongue virus - ویروس Bluetonguepfu - پفوmultiplicity of infection - چندین عفونتKOBAS - کوبا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
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چکیده انگلیسی
Bluetongue virus (BTV) is a member of the genus Orbivirus within the family Reoviridae and causes a non-contagious, insect-transmitted disease in domestic and wild ruminants, mainly in sheep and occasionally in cattle and some species of deer. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine which cellular miRNAs were differentially expressed in primary sheep testicular (ST) cells infected with BTV. A total of 25 known miRNAs and 240 novel miRNA candidates that were differentially expressed in BTV-infected and uninfected ST cells were identified, and 251 and 8428 predicted target genes were annotated, respectively. Nine differentially expressed miRNAs and their mRNA targets were validated by quantitative reverse transcription-polymerase chain reaction. Targets prediction and functional analysis of these regulated miRNAs revealed significant enrichment for several signaling pathways including MAPK, PI3K-Akt, endocytosis, Hippo, NF-kB, viral carcinogenesis, FoxO, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in BTV replication and pathogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 49, April 2017, Pages 256-267
Journal: Infection, Genetics and Evolution - Volume 49, April 2017, Pages 256-267
نویسندگان
Junzheng Du, Shandian Gao, Zhancheng Tian, Shanshan Xing, Dexuan Huang, Guorui Zhang, Yadong Zheng, Guangyuan Liu, Jianxun Luo, Huiyun Chang, Hong Yin,