کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5590950 1570312 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of cpSSR markers for analysis of genetic diversity in Gladiolus cultivars
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Development of cpSSR markers for analysis of genetic diversity in Gladiolus cultivars
چکیده انگلیسی
We report the development of 12 novel microsatellite (SSR) markers isolated from repeat sequences of chloroplast region of Gladiolus. A total of 1188 nucleotide sequences of chloroplast regions of Gladiolus were used to search for simple sequence repeats and 108 sequences were found to contain 124 SSRs. Dinucleotide repeat SSRs were most frequent (94.4%) with the AT/AT (59.7%) repeat motif followed by TA/TA (31.5%). Assessment of genetic diversity among 62 commercially important Gladiolus cultivars was carried out using 12 SSR markers. Out of 12 SSR markers, only six showed polymorphism with allelic variation ranging from 2 to 8. These six SSR markers revealed high genetic diversity (84.6%) across different Gladiolus cultivars. The average value of Polymorphic information content (PIC = 0.32) diversity index (2.03), expected heterozygosity (0.354) and Shannon's information index (0.522) were also significantly high. These markers could be utilized for future breeding programmes, genetic diversity studies, and germplasm management of this important ornamental crop.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Gene - Volume 10, June 2017, Pages 31-36
نویسندگان
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