کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5674599 1408245 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals
چکیده انگلیسی
Clustered regularly-interspaced short palindromic repeat (CRISPR) sequences cooperate with CRISPR-associated (Cas) proteins to form the basis of CRISPR-Cas adaptive immune systems in prokaryotes. For more than 20 years, these systems were of interest only to specialists, mainly molecular microbiologists, who tried to understand the properties of this unique defense mechanism. In 2012, the potential of CRISPR-Cas systems was uncovered and these were presented as genome-editing tools with an outstanding capacity to trigger targeted genetic modifications that can be applied to virtually any organism. Shortly thereafter, in early 2013, these tools were shown to efficiently drive specific modification of mammalian genomes. This review attempts to summarize, in a comprehensive manner, the key events and milestones that brought CRISPR-Cas technology from prokaryotes to mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Trends in Microbiology - Volume 24, Issue 10, October 2016, Pages 811-820
نویسندگان
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