کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5675037 1594209 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selection of differently temporally regulated African swine fever virus promoters with variable expression activities and their application for transient and recombinant virus mediated gene expression
ترجمه فارسی عنوان
انتخاب پروتئین های ویروسی مورفولوژیکی آفریقایی که به طور موقت در طول زمان با فعالیت های بیان متغیر و کاربرد آنها برای بیان ژن موثر بر ویروس گذار و نوترکیب
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
African swine fever virus threatens pig production worldwide due to the lack of vaccines, for which generation of both deletion and insertion mutants is considered. For development of the latter, operational ASFV promoters of different temporal regulation and strengths are desirable. We therefore compared the capacities of putative promoter sequences from p72, CD2v, p30, viral DNA polymerase and U104L genes to mediate expression of luciferase from transfected plasmids after activation in trans, or p30-, DNA polymerase- and U104L promoters in cis, using respective ASFV recombinants. We identified sequences with promoter activities upstream the viral ORFs, and showed that they differ in both their expression intensity regulating properties and in their temporal regulation. In summary, p30 and DNA polymerase promoters are recommended for high level early regulated transgene expression. For late expression, the p72, CD2v and U104L promoter are suitable. The latter however, only if low level transgene expression is aimed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 508, August 2017, Pages 70-80
نویسندگان
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