کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5685054 | 1597929 | 2017 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Individual variability in response to a single sickling event for normal, sickle cell, and sickle trait erythrocytes
ترجمه فارسی عنوان
انحصار فردی در پاسخ به یک رویداد سوسپانسیون تنها برای سلول های نرمال، سیگنال و صفات پاره اریتروسیت ها
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Oxy-HbSCDSCARBCDeoxy-Hbmet-HbRed blood cells - سلولهای قرمز خونAUC - AUCScT - ScT درOXY - اکسیSickle cell disease - بیماری سلول داسی شکلHypoxic conditions - شرایط خفیفSickle cell trait - صفت سلولی صفتarea under the curve - منطقه تحت منحنیHemoglobin - هموگلوبینdeoxygenated hemoglobin - هموگلوبین deoxygenatedOxygenated hemoglobin - هموگلوبین اکسید شدهHydroxyurea - هیدروکسی اورهSickle cell anemia - کم خونی سلولی شکم
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی
Hemoglobin S (Hb-S) polymerization is the primary event in sickle cell disease causing irreversible damage to red blood cell (RBC) membranes over repeated polymerization cycles. A single polymerization triggered by a hypoxic environment was reported to result in reversibly (upon reoxygenation) decreased RBC deformability and increased mechanical fragility (MF). Individualized responses have not been reported, although RBC fragility can vary significantly even among healthy individuals. This study evaluates individual variability in response to a single hypoxia-induced sickling event, through changes in RBC MF. Blood was drawn from 10 normal (AA), 11 sickle cell (SS), and 7 sickle trait (AS) subjects-with Hb-S fraction, osmotic fragility, and medical history also collected. Mechanical stress was applied using a bead mill at 50-Hz oscillation for 0.5-30 minutes. MF profiles here give percent hemolysis upon successive durations of stressing. MF was measured for AA, SS, and AS cells-each equilibrated (1) with air, (2) with nitrogen in an anaerobic chamber, and (3) with air after the hypoxic event. While AA subjects exhibited significantly different changes in fragility upon hypoxia, in all cases there was recovery to close to the initial MF values on reoxygenation. For AS subjects, recovery at reoxygenation was observed only in about half of the cases. Fragility of SS cells increased in hypoxia and decreased with reoxygenation, with significantly variable magnitude of recovery. The variability of response for individual AS and SS subjects indicates that some are potentially at higher risk of irreversible hypoxia-induced membrane damage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 181, March 2017, Pages 96-107
Journal: Translational Research - Volume 181, March 2017, Pages 96-107
نویسندگان
Michael Tarasev, Marina Muchnik, Lucia Light, Kenneth Alfano, Sumita Chakraborty,