کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737492 | 1614716 | 2017 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice
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کلمات کلیدی
mIPSCsI-ONO synthasesZD7288ODQeEPSCseIPSCsPSD95PPRmEPSCsDNQXPTXNOSAMPARaCSFTTXcGMP6,7-dinitroquinoxaline-2,3-dione - 6،7-dinitroquinoxaline-2،3-dioned-AP5 - D-AP5NMDA receptor - NMDA گیرندهα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor - α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptorISI - آی اس آیtetrodotoxin - تترو دوتوکسین long-term potentiation - تقویت درازمدتLTP - تقویت طولانی مدت یا LTP miniature inhibitory postsynaptic currents - جریان ممانعت کننده پسینپتیکEvoked inhibitory postsynaptic currents - جریان های پسینپتیک مهار کننده ای ایجاد می کنندminiature excitatory postsynaptic currents - جریانهای پسینپتیک هیجان انگیز مینیاتوریinter-stimulus interval - فاصله بین محرکSomatosensory cortex - قشر سامهوسنسوریartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیevoked excitatory postsynaptic currents - موجب تحریک جریانهای پسینپتیک هیجانی می شودcyclic guanosine monophosphate - مونوفسفات گوانوزین چرخه ایknock out - ناک اوتpaired-pulse ratio - نسبت پالس زوجNitric oxide - نیتریک اکسیدpostsynaptic density protein 95 - پروتئین چگالی Postynaptic 95Picrotoxin - پیکروتوکسینHCN channel - کانال HCNhyperpolarization-activated cyclic nucleotide-gated channel - کانال نوکلئوتیدی غربالگری فعال cyclic hyperpolarization فعال استGABA - گاباglutamate - گلوتاماتN-methyl-d-aspartate receptor - گیرنده N-methyl-d-aspartate
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice](/preview/png/5737492.png)
چکیده انگلیسی
In hippocampus, two guanylyl cyclases (NO-GC1 and NO-GC2) are involved in the transduction of the effects of nitric oxide (NO) on synaptic transmission. However, the respective roles of the NO-GC isoforms on synaptic transmission are less clear in other regions of the brain. In the present study, we used knock-out mice deficient for the NO-GC1 isoform (NO-GC1 KO) to analyze its role in the glutamatergic and GABAergic neurotransmission at pyramidal neurons in layers II/III of somatosensory cortex. NO-GC1 KO slices revealed reduced frequencies of miniature excitatory- and inhibitory-postsynaptic currents, increased paired-pulse ratios and decreased input-output curves of evoked signals, which indicated the reduction of glutamate and GABA release in NO-GC1 KO mice. The functional changes in NO-GC1 KO mice were caused by the lack of cGMP as they were rescued to WT-like levels by the cGMP analog, 8-Br-PET-cGMP and conversely, mimicked by the NO-GC inhibitor, ODQ, in WT slices. In search of a cGMP target, two blockers of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels (ZD7288 and DK-AH269) reduced glutamate release in WT to the level of NO-GC1 KO mice suggesting HCN channels as possible effectors for presynaptic cGMP enhancing the glutamate release probability. By blocking postsynaptic NMDA receptors, the NMDA receptor-dependent NO signal was shown to be linked to the effect of NO-GC1 on presynaptic GABA release. Of note, the balance between glutamatergic and GABAergic inputs at individual synapses remained unaltered in the NO-GC1 KO mice. In sum, our results indicate a role for cGMP generated by presynaptic localized NO-GC1 to adjust inhibitory and excitatory inputs at individual synapses in the somatosensory cortex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 360, 30 September 2017, Pages 180-189
Journal: Neuroscience - Volume 360, 30 September 2017, Pages 180-189
نویسندگان
Qi Wang, Evanthia Mergia, Doris Koesling, Thomas Mittmann,