کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5745719 1618780 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Subacute oral toxicity assessment of benalaxyl in mice based on metabolomics methods
ترجمه فارسی عنوان
ارزیابی مسمومیت با سم زدایی از بنالاکسیل در موش ها بر اساس روش های متابولومیسم
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی


- Mice were exposed to benalaxyl for 30 consecutive days.
- Metabolic responses were assessed using untargeted and targeted metabolomics approaches.
- Significant oxidative damage was observed, without histopathological injury.
- Distribution of benalaxyl in brain was stereoselective.

In this study, the metabolic responses of mice after 30 days of exposure to benalaxyl were assessed using NMR-based untargeted metabolomics and LC-MS-based targeted profiling of 20 amino acids. Urinary 1H NMR analyses revealed alterations in energy metabolism, lipid metabolism, vitamin B metabolism, the urea cycle and amino acid metabolism, and targeted analyses indicated that the serum levels of asparagine, histidine, lysine and aspartic acid were significantly altered. Additionally, significant oxidative stress was observed in the liver and kidney, although no apparent histopathological injury was observed. The tissue distribution indicated a significant stereoselectivity in the brain, where (−)-R-benalaxyl was enriched. These data provide a comprehensive picture of the subacute toxic effects of benalaxyl in mice. The results of this study suggested that, for a toxicity evaluation, metabolomics analysis is much more sensitive than traditional toxicological methods. The results also highlight the combined use of untargeted and targeted metabolomics approaches in evaluating the health risks of xenobiotics.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 191, January 2018, Pages 373-380
نویسندگان
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