کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5747737 | 1618919 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell cycle arrest mediated by Cd-induced DNA damage in Arabidopsis root tips
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کلمات کلیدی
MLH1PCNACycB1GR1Cyclin B1MSH2qRT-PCRATRDSBFCMataxia-telangiectasia mutated - Ataxia-Telangiectasia جهش یافته استCDKs - CDK هاROS - ROSArabidopsis - آرابیدوپسیس یا رشادیproliferation cell nuclear antigen - آنتی ژن هسته ای سلول تکثیرCd stress - استرس سی دیGene expression - بیان ژنCell cycle arrest - توقف چرخه سلولیATM - خودپردازdouble strand break - شکست دو رشتهFlow cytometry - فلوسیتومتریBRCA1 - ژن BRCA1cyclin-dependent kinases - کیناز وابسته به سیکلینReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Accumulating evidence demonstrates that the aberrant expression of cell cycle regulation and DNA repair genes can result in abnormal cell proliferation and genomic instability in eukaryotic cells under different stresses. Herein, Arabidopsis thaliana (Arabidopsis) seedlings were grown hydroponically on 0.5 à MS media containing cadmium (Cd) at 0-2.5 mg Lâ1 for 5 d of treatment. Real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that expression of DNA damage repair and cell cycle regulation genes, including BRCA1, MRE11, WEE1, CDKA;1 and PCNA1, showed an inverted U-shaped dose-response. In contrast, notably reduced expression was observed for G1-to-S transition-related genes, Histone H4, E2Fa and PCNA2; DSB end processing, GR1; G2-to-M transition-related gene, CYCB1;1; and DNA mismatch repair, MSH2, MSH6 and MLH1 genes in root tips exposed to 0.125-2.5 mg/L Cd for 5 d. Flow cytometry (FCM) analysis revealed significant increases of cells with a 2C nuclear content and with a 4C and 8C nuclear content under Cd stresses of 0.125 and 1-2.5 mg Lâ1, respectively. Our results suggest that 0.125 mg Lâ1 Cd-induced DNA damage induced the marked G1/S arrest, leading to accelerated growth in root tips, while 1.0-2.5 mg Lâ1 Cd-induced DNA damage caused a notable G2/M arrest in root tips, leading to reduced growth in root tips. This may be a protective mechanism that prevents cells with damaged DNA from dividing under Cd stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 145, November 2017, Pages 569-574
Journal: Ecotoxicology and Environmental Safety - Volume 145, November 2017, Pages 569-574
نویسندگان
Weina Cui, Hetong Wang, Jie Song, Xia Cao, Hilary J. Rogers, Dennis Francis, Chunyun Jia, Lizong Sun, Meifang Hou, Yuesuo Yang, Peidong Tai, Wan Liu,