کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5762112 | 1624805 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plant regeneration from ploidy-stable cryopreserved embryogenic lines of the hybrid Pinus elliottii x P. caribaea
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کلمات کلیدی
RAPDFWIWPBMVFMLVECLCryoprotectantPPFDPGRCPPUFCMBAPSSF2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسید6-Benzylaminopurine - 6-بنزیلامینوپورینDMSO - DMSORandomly amplified polymorphic DNA - DNA پلیمورفیک به طور تصادفی تقویت شده استPlant growth regulators - تنظیم کننده های رشد گیاهیGenetic variability - تنوع ژنتیکیSomatic embryogenesis - جنین زودهنگامsomatic embryos - جنین های سمیDimethyl sulfoxide - دیمتیل سولفواکسیدCryopreservation - سرماداری Dispersion index - شاخص پراکندگیCoefficient of Variation - ضریب تغییرphotosynthetic photon flux density - فتوسنتز تراکم شار فوتونFlow cytometry - فلوسیتومتریCSF - مایع مغزی نخاعیfresh weight - وزن ترPropidium iodide - پروتئین یدیدpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Clonal breeding programs of the highly important interspecific hybrid Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis require the establishment of robust cryopreservation protocols, with no genetic variability. Embryonal masses of this hybrid were successfully cryopreserved by a fast slow-freezing method. The protocol was optimized by testing the effects of dimethyl sulfoxide (DMSO) based cryoprotectant solutions, sugar cryoprotectants (e.g. sucrose or maltose, 0.4Â M) and different times of pretreatments and pre-cooling storage. The addition of DMSO in a mixture of polyethylene glycol (PEG) 4000 and sucrose (PSD solution), instead of DMSO alone, was beneficial for recovery of cryopreserved cultures. This parameter, together with the time of pre-cooling storage (before plunging in liquid nitrogen), were the factors that most influenced the survival and regrowth rates of embryonal masses. A pretreatment combination of sucrose (0.4Â M) and 5% PSD followed by a pre-cooling storage of 24Â h allowed the cryopreservation and regrowth of embryogenic cell lines without major genetic variations (DNA-ploidy) or loss of embryogenic potential. Eight of the nine tested embryogenic cell lines survived to the cryopreservation procedures, however the genotype and treatment conditions clearly influenced the response. Somatic embryos maturation and conversion from recovered embryonal masses (EMs) took place according to our standard somatic embryogenesis protocol for Pinus, using modified Litvai (mLV) medium. This is the first report for this hybrid, and the proposed protocol is fast, robust, providing high rates of recovery and working with several genotypes. This protocol's suitability for industrial breeding programmes was also confirmed by allowing the regeneration of plants with no apparent variability from cryopreserved embryonal masses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 105, 15 October 2017, Pages 215-224
Journal: Industrial Crops and Products - Volume 105, 15 October 2017, Pages 215-224
نویسندگان
Sandra Nunes, Liliana Marum, Nelson Farinha, Vanessa Tolentino Pereira, Tânia Almeida, Maria Celeste Dias, Conceição Santos,