کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5762880 | 1625145 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and expression patterns of mannose-binding lectin (MMBL) gene in mulberry (Morus multicaulis) and its prokaryotic expression in E. coli
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کلمات کلیدی
PNPIPTGHRPORFRT-PCRNCBIqRT-PCRGNADEPCMBLPBSDABEULcDNA - cDNADNA - DNA یا اسید دزوکسی ریبونوکلئیکGalanthus nivalis agglutinin - Galanthus nivalis آگلوتیینینp-nitrophenyl - p-نیترفنیلβ-mercaptoethanol - β-merkaptoethanolβ-Me - β-منEST - استdeoxyribonucleic acid - اسید deoxyribonucleiccomplementary deoxyribonucleic acid - اسید دز اکسید ریبونوکلئیک مکملRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدisopropyl β-d-thiogalactoside - ایزوپروپیل β-d-thiogalactosideProkaryotic expression - بیان پروکاریوتیExpression analysis - تجزیه و تحلیل بیانCharacterization - تعیین مشخصاتExpressed sequence tags - تگ های توالی بیان شدهdiethylpyrocarbonate - دیاتیلپیر کربناتdiaminobenzidine - دیامینو بنزیدینMulberry - شاه توتphosphate buffer saline - فسفات بافر شورopen reading frame - قاب خواندن بازLuria-Bertani - لواریا بارتانیMannose-binding lectin - لکتین اتصال دهنده مانوزNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیIsoelectric point - نقطه ایزوالکتریکpolymerase chain reaction - واکنش زنجیره ای پلیمرازquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استPCR - واکنش زنجیرهٔ پلیمرازHorseradish peroxidase - پراکسیداز هوررادیشoptical density - چگالی نوری
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mannose-binding lectin (MBL) is an important natural immune molecule which plays a significant role in plant self-defense and shows a broad spectrum resistance on plant pathogens, viral pathogens, and fungi. In the present paper, a cDNA sequence encoding MBL, which was designated MMBL (GenBank accession NO: KY348866), was cloned from the leaves of mulberry (Morus multicaulis) based on mulberry expressed sequence tags (EST) and homologous cloning technology using RT-PCR. The cDNA was 978Â bp in length with a 5â² untranslated regions (UTR) of 189Â bp, a 3â² UTR of 303Â bp and an open reading frame (ORF) of 486Â bp encoding a protein of 161 amino acids. The estimated molecular weight and isoelectric point (pI) of the putative protein were16.97Â kDa and 6.07, respectively. The MMBL had Jacalin domain and six sugar binding sites, and belonged to Jacalin-like superfamily. Phylogenetic analysis based on the amino acid sequences encoded by the MBL gene from various species showed that mulberry was closely related to Morus notabilis, Ipomoea nil, Capsicum annuum, Solanum lycopersicum and Solanum tuberosum. Quantitative real-time PCR (qRT-PCR) analysis revealed that MMBL was expressed in all the tested tissues, including leaf, bud, fruit, stem, phloem and xylem of the mulberry with the highest expression in the bud and leaf. The expression level of the mRNA has changed significantly under drought, cold, salt and mechanical damage stress treatments compared to the normal growth environment. The ORF segment of the MMBL was inserted into the expression plasmid pET-28a(+) to construct a recombinant expression plasmid. SDS-PAGE and western blot results revealed that His-tagged fusion protein was successfully expressed. Overall, these results showed a better understanding of the molecular basis for the signal transduction mechanism during the stress responses in mulberry trees.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: South African Journal of Botany - Volume 113, November 2017, Pages 1-10
Journal: South African Journal of Botany - Volume 113, November 2017, Pages 1-10
نویسندگان
R. Li, T. Wang, D. Chen, K. Dominic, R. Li, Y. Wang, L. Liu, W. Zhao,