کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5767513 | 1628386 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Biofilm is a serious problem in food industry.
- L. monocytogenes can develop biofilm.
- ListShieldTM can reduce L. monocytogenes in biofilms.
Listeria monocytogenes can persist and form biofilms in a food environment which are difficult to eradicate because biofilms are inherently resistant to a variety of antimicrobial treatments. Therefore, alternative approaches such as bacteriophages have been suggested as a promising biocontrol agent against biofilms. The aim of this study was to evaluate the efficacy of a cocktail bacteriophage product (ListShieldâ¢) against L. monocytogenes biofilms. These biofilms were established on lettuce, stainless steel, rubber, and a MBEC biofilm device and exposed to the ListShield⢠phage preparation (1 Ã 108 PFU/mL) for 2 h. ListShield⢠had sufficient potency to significantly reduce the biofilm (P < 0.05) in all cases. Biofilm reduction achieved after ListShield⢠treatment on the stainless steel coupon was 1.9-2.4 log CFU/cm2 and on the rubber surface approximately 1.0 log CFU/cm2. Phage application on lettuce inactivated biofilm bacteria up to 0.7 log CFU/cm2. These results suggest that bacteriophage preparation ListShield⢠is an effective tool for the inactivation of L. monocytogenes biofilms in the food industry.
Journal: Food Control - Volume 78, August 2017, Pages 256-263