کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5799823 1555344 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding of Clostridium perfringens to collagen correlates with the ability to cause necrotic enteritis in chickens
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Binding of Clostridium perfringens to collagen correlates with the ability to cause necrotic enteritis in chickens
چکیده انگلیسی


- Clostridium perfringens strains with known ability to cause necrotic enteritis were tested in a collagen adherence assay.
- The same strains were screened for the adhesin gene of VR-10B; cnaA.
- Both the presence of cnaA and the ability to adhere to collagen strongly correlated with the ability of a strain to cause necrotic enteritis.
- The product of cnaA may be playing a role in adherence to collagen types IV, V and gelatin.

This study investigated the ability of Clostridium perfringens isolates derived from chickens to bind to collagen types I-V and gelatin. In total 21 strains from three distinct backgrounds were studied: (i) virulent strains isolated from birds suffering from necrotic enteritis, (ii) avirulent strains isolated from birds suffering from necrotic enteritis and (iii) strains isolated from healthy birds. All strains isolated from diseased birds had been assessed for virulence in a disease induction model. The virulent isolates all displayed collagen binding ability. However, most strains in the other two classes showed negligible binding to collagen. The prevalence of a previously described C. perfringens putative collagen adhesin-encoding gene was investigated by PCR screening. It was found that five of the strains carried the putative collagen adhesin-encoding gene and that all of these strains were virulent isolates. Based on these studies it is postulated that collagen adhesion may play a role in the pathogenesis of necrotic enteritis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 180, Issues 3–4, 18 November 2015, Pages 299-303
نویسندگان
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