کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5800171 | 1555346 | 2015 | 8 صفحه PDF | دانلود رایگان |
- Three PRRSV isolates had highest nucleotide identity with vaccine strain JXA1-P80.
- The three isolates shared similar phylogenetic properties with JXA1-P80.
- A total of 12 amino acids are only common to the isolates and attenuated JXA1s.
- Infection experiments showed that the three PRRSV isolates were highly pathogenic.
- The three isolates are likely to be revertants of the live vaccine JXA1-P80.
Three porcine reproductive and respiratory syndrome viruses (PRRSV), NT1, NT2, and NT3, were isolated from three dying piglets from a single pig farm in Jiangsu Province, China. Whole genome sequencing revealed that the three isolates share the highest homology with JXA1-P80, an attenuated vaccine strain developed by serial passage of highly pathogenic PRRSV JXA1 in MARC-145 cells. More than ten amino acids residues in ORF1a, ORF1b, GP4, and GP5 that were thought to be unique to JXA1 attenuated on MARC-145 cells were each found in the corresponding locations of NT1, NT2, and NT3. In virulence assays, piglets infected with NT1, NT2, or NT3 exhibited clinical signs of disease, including high fever, anorexia, and respiratory distress, leading to the death of the majority of the piglets within two weeks. Collectively, these data indicate that NT1, NT2, and NT3 are highly pathogenic PRRSVs and they are likely to be revertants of the vaccine strain JXA1-P80.
Journal: Veterinary Microbiology - Volume 179, Issues 3â4, 30 September 2015, Pages 242-249