کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5800967 | 1555368 | 2013 | 8 صفحه PDF | دانلود رایگان |
Ovine brucellosis caused by Brucella ovis is considered one of the most important reproductive diseases of rams worldwide. This study aimed to characterize the kinetics of infection of a ÎabcAB B. ovis mutant strain in rams. Twelve 1-year-old crossbred rams were used. Six rams were challenged with 2 mL of a suspension containing 1.2 Ã 109 CFU/mL of B. ovis strain ATCC25840 (wild type) by intraprepucial inoculation and additional 50 μL in each conjunctival sac of a suspension containing 1.2 Ã 1010 CFU/mL of the same strain. The other six rams were challenged with an equivalent number of CFU of the mutant strain ÎabcAB B. ovis through the same routes. Serum samples for serology and semen and urine samples for bacteriologic culture and PCR were collected weekly during 24 weeks. At 24 weeks post infection, tissue samples were collected for bacteriologic culture and PCR. All rams inoculated with wild type or the ÎabcAB strain seroconverted at the fourth week post infection, remaining positive up to the 16th week post infection. PCR and bacteriology demonstrated that only rams inoculated with the wild type strain shed the organism in semen and urine. Lymphocytes from rams inoculated with wild type or ÎabcAB B. ovis had significantly higher proliferation in response to B. ovis antigens when compared with unstimulated controls. Tissue bacteriology and PCR detected B. ovis in all rams challenged with the wild type strain, whereas only one ÎabcAB-infected ram had a positive iliac lymph node sample by PCR.
Journal: Veterinary Microbiology - Volume 167, Issues 3â4, 27 December 2013, Pages 546-553