کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5801007 | 1555378 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in England and Wales since 2001
ترجمه فارسی عنوان
افزایش تنوع فیلوژنیک ویروس اسهال ویروس گاو نوع 1 در انگلستان و ویلز از سال 2001
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کلمات کلیدی
ویروس اسهال ویروس گاو، 5؟ ² منطقه غیر ترجمه، منطقه نپرو، تجزیه و تحلیل فیلوژنتیک،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Currently, there are two recognised genotypes of Bovine viral diarrhoea virus (BVDV), type 1 and type 2. These genotypes are divided into subtypes based on phylogenetic analysis, namely a-p for BVDV-1 and a-c for BVDV-2. Within this study, the genetic heterogeneity of BVDV-1 in England and Wales was investigated and compared to the situation in 1996/1997. Viral RNA was extracted from 316 blood samples collected between 2004 and 2009 that were previously identified as BVDV-1 positive. A region of the 5â² untranslated region (UTR) was amplified by RT-PCR and the PCR products were sequenced. Phylogenetic analysis of the 5â²UTR demonstrated the existence of five subtypes of BVDV-1 circulating in England and Wales, namely BVDV-1a (244 samples), BVDV-1b (50), BVDV-1e (3), BVDV-1f (1) and BVDV-1i (18). Phylogenetic analysis of the nucleotide sequence for the Npro region of the viral genome supported the classification obtained with the 5â²UTR. Given the fact that only three subtypes were detected in 1999 this report supports the notion that the restocking of cattle from continental Europe, after the mass culling during the Foot-and-Mouth outbreak in 2001 and slaughter of cattle due to bovine tuberculosis infection, has increased the genetic diversity of BVDV-1 subtypes in England and Wales in the past 10Â years.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 162, Issues 2â4, 23 March 2013, Pages 315-320
Journal: Veterinary Microbiology - Volume 162, Issues 2â4, 23 March 2013, Pages 315-320
نویسندگان
R. Strong, J. Errington, R. Cook, N. Ross-Smith, P. Wakeley, F. Steinbach,