کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5814612 | 1556635 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Type I interferons up-regulate the expression and signalling of p75 NTR/TrkA receptor complex in differentiated human SH-SY5Y neuroblastoma cells
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کلمات کلیدی
PI3KTNFtrkAGAP-43PARPp75NTRPLCFCSNGFEAEPMSFDAPIGAPDHPBSSDSJnkNGSBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز c-Jun N-terminal kinase - C-Jun N-terminal kinaseERK1/2 - ERK1 / 2MAPK - MAPKSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNASTAT - آمارexperimental allergic encephalomyelitis - آنسفالومیلیت آلرژیک تجربیNeuronal apoptosis - آپوپتوز عصبیall-trans-retinoic acid - اسید کلرید رتینوئیکinterferon - اینترفرونIFN - اینترفرون هاType I interferons - اینترفرون های نوع ICNS - دستگاه عصبی مرکزیgrowth associated protein-43 - رشد پروتئینی مرتبط با آن 43sodium dodecyl sulphate - سدیم دودسیل سولفاتnormal goat serum - سرم طبیعی بزfetal calf serum - سرم گوساله جنینcentral nervous system - سیستم عصبی مرکزیnerve growth factor - فاکتور رشد عصبBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزtumor necrosis factor - فاکتور نکروز تومورPhosphate buffered saline - فسفات بافر شورphospholipase C - فسفولیپاز Cphosphoinositide 3-kinase - فسفینوزیتید 3-کینازphenylmethylsulphonyl fluoride - فنیل متیل سولفونیل فلورایدSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)Neurotrophins - نوروتروفین Signal transduction - هدایت سیگنالextracellular signal-regulated protein kinases 1 and 2 - پروتئین کیناز 1 و 2 تنظیم شده توسط سیگنال خارج سلولیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Type I interferons up-regulate the expression and signalling of p75 NTR/TrkA receptor complex in differentiated human SH-SY5Y neuroblastoma cells Type I interferons up-regulate the expression and signalling of p75 NTR/TrkA receptor complex in differentiated human SH-SY5Y neuroblastoma cells](/preview/png/5814612.png)
چکیده انگلیسی
Both type I interferons (IFNs) and neurotrophins regulate neuroadaptive responses, but relatively little is known on the interaction between these two classes of regulatory proteins. Here we investigated the effect of IFN-β on the expression and functional activity of the common neurotrophin receptor p75NTR and the nerve growth factor (NGF) receptor TrkA. In differentiated human SH-SY5Y neuroblastoma cells prolonged exposure to IFN-β up-regulated p75NTR and TrkA levels, failed to affect the content of sortilin, a p75NTR co-receptor, and, consistent with our previous finding, down-regulated the brain-derived neurotrophic factor receptor TrkB. Quantitative real time RT-PCR indicated that IFN-β increased p75NTR and TrkA mRNA levels. In control and IFN-β treated cells proNGF failed to induce c-Jun N-terminal kinase and nuclear factor/kB activation, two p75NTR/sortilin signalling pathways mediating neuronal death. On the other hand, IFN-β treatment enhanced TrkA autophosphorylation and signalling induced by NGF and proNGF. Knockdown of p75NTR by siRNA reduced TrkA activation by proNGF and a subnanomolar concentration of NGF, whereas co-immunoprecipitation indicated close association of p75NTR and TrkA. Co-treatment with either NGF or proNGF reduced IFN-β pro-apoptotic and anti-neurotrophic effects. Similarly, in primary mouse hippocampal neurons IFN-β increased p75NTR and TrkA expression, down-regulated TrkB and enhanced NGF-induced phosphorylation of the pro-survival protein kinase Akt. The data demonstrate that in neuronal cells IFN-β differentially affects the expression and signalling of neurotrophin receptors and suggest that the up-regulation of the p75NTR/TrkA signalling complex may constitute a novel mechanism by which this cytokine selectively attenuates its pro-apoptotic effect in NGF-responsive cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 79, April 2014, Pages 321-334
Journal: Neuropharmacology - Volume 79, April 2014, Pages 321-334
نویسندگان
Simona Dedoni, Maria C. Olianas, Angela Ingianni, Pierluigi Onali,