کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5820806 | 1557399 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells
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کلمات کلیدی
DMEMapoprotein BapoBHYPPBSFBSCCDDMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoEDTA - اتیلن دی آمین تترا استیک اسید ethylene diaminetetraacetic acid - اتیلن دیامین تتراستیک اسیدFörster resonance energy transfer - انتقال انرژی تابشی ForsterFRET - انتقال انرژی رزونانسی فورسترDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوmononuclear phagocytic system - سیستم فاگوسیتیک تک هسته ایcharge coupled device - شارژ دستگاه همراهLDL - لیپوپروتئین کم چگالی(کلسترول بد)low-density lipoproteins - لیپوپروتئینهای کم چگالیMPs - نمایندگان مجلسHypericin - هیپریکین
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
علوم دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells](/preview/png/5820806.png)
چکیده انگلیسی
Low-density lipoproteins (LDL), a natural in vivo carrier of cholesterol in the vascular system, play a key role in the delivery of hydrophobic/amphiphilic photosensitizers to tumor cells in photodynamic therapy of cancer. To make this delivery system even more efficient, we have constructed a nano-delivery system by coating of LDL surface by dextran. Fluorescence spectroscopy, confocal fluorescence imaging, stopped-flow experiments and flow-cytometry were used to characterize redistribution of hypericin (Hyp), a natural occurring potent photosensitizer, loaded in LDL/dextran complex to free LDL molecules as well as to monitor cellular uptake of Hyp by U87-MG cells. It is shown that the redistribution process of Hyp between LDL molecules is significantly suppressed by dextran coating of LDL surface. The modification of LDL molecules by dextran does not inhibit their recognition by cellular LDL receptors and U-87 MG cellular uptake of Hyp loaded in LDL/dextran complex appears to be similar to that one observed for Hyp transported by unmodified LDL particles. Thus, it is proposed that dextran modified LDL molecules could be used as a basis for construction of a drug transport system for targeted delivery of hydrophobic/amphiphilic drugs to cancer cells expressing high level of LDL receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 436, Issues 1â2, 15 October 2012, Pages 463-471
Journal: International Journal of Pharmaceutics - Volume 436, Issues 1â2, 15 October 2012, Pages 463-471
نویسندگان
Veronika Huntosova, Diana Buzova, Dana Petrovajova, Peter Kasak, Zuzana Nadova, Daniel Jancura, Franck Sureau, Pavol Miskovsky,