کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5822215 1557838 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of replication of porcine reproductive and respiratory syndrome virus by hemin is highly dependent on heme oxygenase-1, but independent of iron in MARC-145 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Inhibition of replication of porcine reproductive and respiratory syndrome virus by hemin is highly dependent on heme oxygenase-1, but independent of iron in MARC-145 cells
چکیده انگلیسی
Current vaccines against porcine reproductive and respiratory syndrome virus (PRRSV) have failed to provide sustainable disease control, and development of new antiviral strategies is of great importance. The present study investigated the mechanism of the antiviral effect of hemin during PRRSV infection in MARC-145 cells. Hemin, a commercial preparation of heme, is used as an iron donor or heme oxygenase 1 (HO-1) inducer, and has been shown to provide antiviral activity in many studies. In the current study, the anti-PRRSV activity of hemin was identified through suppressing PRRSV propagation. The 50% inhibitory concentration (IC50) of hemin antiviral activity was estimated to be 32 μM, and the 50% cytotoxic concentration (CC50) of hemin was found to be higher than 125 μM. Further study showed that the antiviral activity of hemin is independent of iron. In addition, after treatment with Protoporphyrin IX zinc (II) (ZnPP) or Sn (IV) Protoporphyrin IX dichloride (SnPP), inhibitors of HO-1, the inhibition of viral replication by hemin was partially reversed. Additionally, it was confirmed that hemin and N-acetyl cysteine were able to significantly reduce reactive oxygen species (ROS) in MARC-145 cells infected with virus. N-acetyl-L-cysteine (NAC), however, did not produce a reduction in viral load or promote expression of HO-1. Taken together, these data indicate that the effect of hemin on the inhibition of PRRSV propagation via HO-1 induction, as well as the antiviral mechanism of HO-1, is not dependent on decreased levels of ROS. In conclusion, these data demonstrate that hemin had antiviral activity against PRRSV and may serve as a useful antiviral agent inhibiting PRRSV replication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 105, May 2014, Pages 39-46
نویسندگان
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