کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5822384 | 1117940 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection
ترجمه فارسی عنوان
خنثی سازی ویروس دنگی و فعالیت های تقویت آنتی بادی وابسته به آنتی بادی های مونوکلونال انسانی مشتق شده از بیماران دنگی در مرحله حاد عفونت ثانویه
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کلمات کلیدی
ویروس دنگی تب دنگی، تب خونریزی دهنده دنگ، آنتی بادی منوکلونال انسانی، خنثی سازی، افزایش وابستگی آنتی بادی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
Public health concern about dengue diseases, caused by mosquito-borne infections with four serotypes of dengue virus (DENV-1-DENV-4), is escalating in tropical and subtropical countries. Most of the severe dengue cases occur in patients experiencing a secondary infection with a serotype that is different from the first infection. This is believed to be due to antibody-dependent enhancement (ADE), by which one DENV serotype uses pre-existing anti-DENV antibodies elicited in the primary infection to facilitate entry of a different DENV serotype into the Fc receptor-positive macrophages. Recently, we prepared a number of hybridomas producing human monoclonal antibodies (HuMAbs) by using peripheral blood lymphocytes from Thai patients at acute phase of secondary infection with DENV-2. Here, we characterized 17 HuMAbs prepared from two patients with dengue fever (DF) and one patient with dengue hemorrhagic fever (DHF) that were selected as antibodies recognizing viral envelope protein and showing higher neutralization activity to all serotypes. In vivo evaluation using suckling mice revealed near perfect activity to prevent mouse lethality following intracerebral DENV-2 inoculation. In a THP-1 cell assay, these HuMAbs showed ADE activities against DENV-2 at similar levels between HuMAbs derived from DF and DHF patients. However, the F(ab')2 fragment of the HuMAb showed a similar virus neutralization activity as original, with no ADE activity. Thus, these HuMAbs could be one of the therapeutic candidates against DENV infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 98, Issue 3, June 2013, Pages 423-431
Journal: Antiviral Research - Volume 98, Issue 3, June 2013, Pages 423-431
نویسندگان
Tadahiro Sasaki, Chayanee Setthapramote, Takeshi Kurosu, Mitsuhiro Nishimura, Azusa Asai, Magot D. Omokoko, Chonlatip Pipattanaboon, Pannamthip Pitaksajjakul, Kriengsak Limkittikul, Arunee Subchareon, Panjaporn Chaichana, Tamaki Okabayashi, Itaru Hirai,