کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5823897 | 1118373 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Uncoupling of K+ and Clâ transport across the cell membrane in the process of regulatory volume decrease
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
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چکیده انگلیسی
It is accepted that K+ and Clâ flows are coupled tightly in regulatory volume decrease (RVD). However, using self referencing microelectrodes, we proved that K+ and Clâ transport mainly by channels in RVD was uncoupled in nasopharyngeal carcinoma CNE-2Z cells, with the transient K+ efflux activated earlier and sustained Clâ efflux activated later. Hypotonic challenges decreased intracellular pH (pHi), and activated a proton pump-dependent H+ efflux, resulting in a decline of extracellular pH (pHo). Modest decreases of pHo inhibited the volume-activated K+ outflow and RVD, but not the Clâ outflow, while inhibition of H+ efflux or increase of pHo buffer ability promoted K+ efflux and RVD. The results suggest that the temporal dynamics of K+ channel activities is different from that of Clâ channels in RVD, due to differential sensitivity of K+ and Clâ channels to pHo. H+ efflux may play important roles in cell volume regulation, and may be a therapeutic target for human nasopharyngeal carcinoma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 84, Issue 3, 1 August 2012, Pages 292-302
Journal: Biochemical Pharmacology - Volume 84, Issue 3, 1 August 2012, Pages 292-302
نویسندگان
Linjie Yang, Linyan Zhu, Yue Xu, Haifeng Zhang, Wencai Ye, Jianwen Mao, Lixin Chen, Liwei Wang,