کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5846184 | 1128461 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
GCLCNrf2GCLMGSHGSTGSSGkeap1 - buy1ROS - ROSElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزAntioxidant enzymes - آنزیم های آنتی اکسیدانAndrographolide - اندوگرافولیدHepatoprotection - حفاظت از هپاتیتSOD - سدEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکSuperoxide dismutase - سوکسوکس دیسموتازantioxidant response element - عنصر پاسخ آنتی اکسیدانnuclear factor erythroid 2-related factor 2 - فاکتور هسته ای عامل erythroid 2 مرتبط 2Bioavailability - فراهم زیستیthiobarbituric acid-reactive substances - مواد واکنش پذیر اسید تیوباربیتوریکARE - هستندheme oxygenase - همگام اکسژنازKelch-like ECH-associated protein 1 - پروتئین مرتبط با ECH کلچ 1Carbon tetrachloride - کربن تتراکلریدglutamate cysteine ligase modifier subunit - کلرید کلسیم،Glutamate cysteine ligase catalytic subunit - کلوتامین کاتالیزوری لیستاز سیستئین گلوتاماتGlutathione - گلوتاتیونglutathione S-transferase - گلوتاتیون S-ترانسفرازglutathione disulfide - گلوتاتیون دی سولفیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Andrographolide, a bioactive diterpenoid, is identified in Andrographis paniculata. In this study, we investigated the pharmacokinetics and bioavailability of andrographolide in rats and studied whether andrographolide enhances antioxidant defense in a variety of tissues and protects against carbon tetrachloride-induced oxidative damage. After a single 50-mg/kg administration, the maximum plasma concentration of andrographolide was 1 μM which peaked at 30 min. The bioavailability of andrographolide was 1.19%. In a hepatoprotection study, rats were intragastrically dosed with 30 or 50 mg/kg andrographolide for 5 consecutive days. The results showed that andrographolide up-regulated glutamate cysteine ligase (GCL) catalytic and modifier subunits, superoxide dismutase (SOD)-1, heme oxygenase (HO)-1, and glutathione (GSH) S-transferase (GST) Ya/Yb protein and mRNA expression in the liver, heart, and kidneys. The activity of SOD, GST, and GSH reductase was also increased in rats dosed with andrographolide (p < 0.05). Immunoblot analysis and EMSA revealed that andrographolide increased nuclear Nrf2 contents and Nrf2 binding to DNA, respectively. After the 5-day andrographolide treatment, one group of animals was intraperitoneally injected with carbon tetrachloride (CCl4) at day 6. Andrographolide pretreatment suppressed CCl4-induced plasma aminotransferase activity and hepatic lipid peroxidation (p < 0.05). These results suggest that andrographolide is quickly absorbed in the intestinal tract in rats with a bioavailability of 1.19%. Andrographolide protects against chemical-induced oxidative damage by up-regulating the gene transcription and activity of antioxidant enzymes in various tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 280, Issue 1, 1 October 2014, Pages 1-9
Journal: Toxicology and Applied Pharmacology - Volume 280, Issue 1, 1 October 2014, Pages 1-9
نویسندگان
Haw-Wen Chen, Chin-Shiu Huang, Chien-Chun Li, Ai-Hsuan Lin, Yu-Ju Huang, Tsu-Shing Wang, Hsien-Tsung Yao, Chong-Kuei Lii,