کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5848187 1130141 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA binding and oxidative DNA damage induced by climacostol-copper(II) complexes: Implications for anticancer properties
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
DNA binding and oxidative DNA damage induced by climacostol-copper(II) complexes: Implications for anticancer properties
چکیده انگلیسی


- The protozoan toxin climacostol exerts prooxidant activity in the presence of Cu(II) ions.
- Climacostol binds to DNA.
- The prooxidant activity can damage plasmid and eukaryotic DNA.

Climacostol is a natural toxin isolated from the freshwater ciliated protozoan Climacostomum virens and belongs to the group of resorcinolic lipids. Climacostol exerts a potent antimicrobial activity against a panel of bacterial and fungal pathogens. In addition it inhibits the growth of tumor cell lines in a dose-dependent manner by inducing programmed cell death via intrinsic pathway. In this work, we investigated the possibility that climacostol exerts a prooxidant effect, inducing plasmid DNA strand breakage and eukaryotic DNA damage in presence of Cu(II) ions. Inhibition of DNA breakage using SOD, catalase and neocuproine confirmed the involvement of reactive oxygen species and Cu(I) ions in the DNA damage. UV-visible absorption changes and mass spectrometric analysis identified a product of reaction as a deprotonated form of climacostol. Study of the interaction with DNA, using fluorescence spectroscopic techniques, showed that climacostol binds with DNA. Given the structure-activity relationship of this compound and the mechanism of its prooxidant effect, we propose that the Cu(II)-mediated oxidative DNA damage by climacostol could explain its antimicrobial and antiproliferative activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 206, Issue 1, 25 October 2013, Pages 109-116
نویسندگان
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